Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis

Figure 2

Pretreatment with G-CSF promotes the migration of BM-MSCs to injured lung tissue. Mice received a tail vein injection of GFP-labelled BM-MSCs on day 14, and a single-cell suspension was generated from lung tissue for FCM analysis on day 21 (a, b). (a) Representative FCM analysis of GFP-labelled BM-MSC population in lung tissue. (b) Data analysis of GFP-labelled BM-MSCs population tested by FCM. In the transwell experiment, after allowing the BM-MSCs to migrate for 18 hours, the number of BM-MSCs was calculated under the microscope (c, d). (c) Representative images of migrated BM-MSCs (crystal violet staining: purple). (d) Data analysis of migrated BM-MSCs which are marked with arrows. BM-MSCs(1) = 1 × 10⁵ cells, BM-MSCs(2) = 3 × 10⁵ cells, and BM-MSCs(3) = 1 × 10⁶ cells. G-CSF>BM-MSCs represented BM-MSCs pretreated with G-CSF (30 ng/mL). –5, . Bars: mean ± SD.

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