Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis

Figure 4

Knockdown of CXCR4 expression reduces the effect of G-CSF-pretreated BM-MSCs on migrating to injured lung tissue. (a) Screening test of CXCR4-RNAi targets. After stably transfecting BM-MSCs with CXCR4-RNAi for 120 h, the mRNA expression of CXCR4 was quantified by real-time PCR. (b) The BM-MSCs transfected with control RNAi or CXCR4-RNAi were treated with or without G-CSF for 24 h; the mRNA expression of CXCR4 was determined by real-time PCR. In the transwell experiment, after allowing the BM-MSCs to migrate for 18 hours, the number of BM-MSCs was calculated under the microscope (c, d). (c) The representative images of migrated BM-MSCs (crystal violet staining: purple). (d) Data analysis of migrated BM-MSCs which are marked with arrows. G-CSF>BM-MSCs represented BM-MSCs pretreated with G-CSF (30 ng/mL). , , . Bars: mean ± SD.

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