Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia

Figure 2

Proliferative and clonogenic potential of MenSCs-PE. (a) Proliferative capacity of MenSCs isolated from healthy women (control) and women who had a past history of preeclampsia (PE). Cell proliferation was evaluated at days 3, 6, and 9 by the SRB assay. MenSCs-PE showed a similar proliferation rate when compared to MenSCs isolated from healthy women. (b) Stromal progenitor capacity; serial dilutions of a defined number of cells in passage 0 (P0) were cultured, and the potential to form stromal progenitors was evaluated. MenSCs-PE show less initial frequency of stromal progenitors than do MenSCs from control women. (c) CFU capacity; serial dilutions of a defined number of cells in passage 4 (P4) were cultured, and the potential to form CFU was evaluated. MenSCs-PE show less frequency of CFU capacity after cell expansion in low dilutions. (d) Representative images of CFU after cell expansion in low dilutions of MenSCs-PE and healthy women (control) cultured at P4. Results are the mean + SEM (standard error of the mean). Statistical analysis was performed using Student’s -tests. and significant.

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