Research Article
Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells
Figure 2
Neuronal differentiation of iPSC-aNPCs. Representative images of clones from iPSC-aNPCLow and iPSC-aNPCHigh subjected to differentiation on PA6 stromal cells stained for (a) TUJ1 and SYP (arrowheads) for immature neuron and presynaptic markers and for MAP2 and TH for mature neuron and dopaminergic neuron markers, respectively. Scale bar represents 100 μm. Insets show higher magnifications. (b) Quantification of marker-positive colonies as a percentage of Hoechst-positive colonies for TUJ1 ( value: 15.56; value < 0.0001), SYP ( value: 18.14; value < 0.0001), MAP2 ( value: 29.13; value < 0.0001), and TH ( value: 61.4; value < 0.0001). Bar shows for four independent experiments. , , and .
| (a) |
| (b) |