Pancreatic Ductal Organoids React Kras Dependent to the Removal of Tumor Suppressive Roadblocks

Figure 1

Pancreas mouse organoids exhibit ductal origin. (a) Organoid size and numbers are depending on the age of the host, with decreased numbers and size when generated from eight compared to two months old WT mice. Organoids shown are low passages 5-7 (40x magnification). (b) Organoids are positive for CK19, FOXA2, and SOX9 (200x magnification). (c) Relative gene expression levels of ductal markers (CK19 and SOX9) and lack of expression of acinar and islet cell markers amylase and PDX1. Gene expression is normalized to HMBS. (d) Ki-67 immunofluorescence (200x magnification). (e) 49.7% of the cells are positive for Ki-67 in WT organoids.

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