Research Article

Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa

Figure 5

Luciferase reporter analysis of the porcine OCT4 regulatory regions in mouse E14 ES cells, mouse P19 cells, and mouse embryonic fibroblasts. (a) Four constructs containing the different elements of the OCT4 promoter in the pGL3-basic vector were transfected into mouse E14 ES cells, mouse P19 cells, and mouse embryonic fibroblasts. Luciferase activity was measured 48 h after transfection. Different letters indicate significant differences (). Error bars indicate the standard error of the means. (b) Reporter gene expression is tabulated on the right (compared to control): +++, high expression; ++, reduced expression; +, greatly reduced expression; and –, lack of expression in each of the different pluripotent-state cultured cell types, E14TG2a mouse embryonic stem cells, P19 mouse embryonic carcinoma cells, and mouse embryonic fibroblasts. OCT4-Luc: conserved regions 1, 2, 3, and 4 (CR1, CR2, CR3, and CR4); DE-Luc: CR1 and CR4; PE-Luc: CR1, CR2, and CR3; CP-Luc: CR1; pGL3-basic: control.
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