Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa

Figure 6

Comparison of naïve- or primed-state-related gene expression between low- and high-fluorescent cells sorted by fluorescence-activated cell sorting. Comparison of marker gene expression from naïve (Tbx3, Nr0b1, Rex1, Esrrb, Nanog, and Klf2) and primed (Gata6, Mixl1, Fgf5, and Otx2) cells using a dual-reporter assay consisting of DE-GFP (pOCT4-∆PE-eGFP) and PE-RFP (pOCT4-∆DE-DsRed2). (a) Embryonic stem cells sorted by the DE-GFP signal. Low GFP/high GFP means that the expression level of genes in GFP-low-expressing cells is divided by that in GFP-high-expressing cells. (b) Embryonic stem cells sorted by the PE-RFP signal. Low RFP/high RFP means that the expression level of genes in RFP-low-expressing cells is divided by that in RFP-high-expressing cells. (c) Embryonic carcinoma cells sorted by the DE-GFP signal. High GFP/low GFP means that the expression level of genes in GFP-high-expressing cells is divided by that in GFP-low-expressing cells. (d) Embryonic carcinoma cells sorted by the PE-RFP signal. High RFP/low RFP means that the expression level of genes in RFP-high-expressing cells is divided by that in RFP-low-expressing cells. The asterisk indicates that no values were observed.

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