Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D

Figure 1

Characterization of MSCs, in comparison with fibroblasts. (a) Determination of cell viability estimated at passage 0 ( for BM- and WJ-MSCs, for DP- and AT-MSCs, and for fibroblasts). (b) Quantification of colony-forming unit fibroblasts (CFU-Fs) evaluated at passage 1 ( for BM-, WJ-, DP-, and AT-MSCs and for fibroblasts). The cell proliferation was estimated by determining the cumulative population doubling levels (c) at passage 5 and (d) at passage 10, as indicated ( for BM-MSCs, for WJ-MSCs, for DP-MSCs, for AT-MSCs, and for fibroblasts). Data are presented as box plots with median as a bar. Statistically significant differences (, , and ).

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