Research Article

Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D

Figure 4

Effect of culture conditions on the mRNA steady-state levels of gene markers of chondrocyte differentiation. Pellets of MSCs, fibroblasts, and nasal chondrocytes were cultivated for 21 days under serum-free conditions in control medium (as indicated by -) or in chondrogenic medium containing BTβ3 or BIT cocktail (as indicated by +). The values obtained in pellets are expressed relative to P1 cells amplified in monolayer under serum-free conditions and used as a reference before differentiation (day 0, reference ). Data are presented as box plots with median as a bar (). Statistically significant differences between BTβ3- and BIT-treated cells and control cells (, , and ). Statistically significant differences between cells cultivated in pellets and day 0 cells (, , and ).
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