Stem Cells International / 2019 / Article / Fig 5

Research Article

The Role of CDR1as in Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells

Figure 5

Knockdown of CDR1as inhibited the differentiation of hucMSCs. (a) The expression levels of STFs in hucMSCs transfected with siRNA or control. (b) The expression levels of STFs in 293 T cells transfected with siRNA or control. (c) Western blot analysis for the expression of Oct4, Lin28, Nanog, Klf4, and Sox2 in hucMSCs transfected with siRNA or control for 24 h, 48 h, and 72 h, respectively. (d) Adipogenic differentiation of hucMSCs after transfection with siRNA or control. (e) Osteogenic differentiation of hucMSCs after transfection with siRNA or control. (f) RT-qPCR for detecting the expression levels of OCN and ALP. ns: no significance; ; ; .
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