Research Article
The Manufacture of GMP-Grade Bone Marrow Stromal Cells with Validated In Vivo Bone-Forming Potential in an Orthopedic Clinical Center in Brazil
Figure 3
In vitro osteogenic, adipogenic, and chondrogenic potential of BMSC products. Representative images of (a) a noninduced BMSC layer, (b) mineralized nodules visualized by Von Kossa staining, and (c) intracellular lipid accumulation stained with Oil Red O. (d–i) Chondrogenic differentiation: (d) H&E staining, (e, f) Masson Trichrome staining, and (g-i) immunofluorescence staining of collagen II in representative BMSC micromass pellet cultures with differentiation towards the chondrogenic lineage. Cartilage matrix deposition (blue) in the extracellular matrix was assessed by Masson Trichrome staining and confirmed by immunofluorescence staining for collagen II. Representative images of experiments. For the immunofluorescence analysis, .
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