The Manufacture of GMP-Grade Bone Marrow Stromal Cells with Validated In Vivo Bone-Forming Potential in an Orthopedic Clinical Center in Brazil

Table 4

Purity and safety analysis of the BMSCs.

	Anaerobic and aerobic bacteria
BMSC 05	ND	0.57	<0.005	7.43
BMSC 06	ND	0.43	<0.005	0.04
BMSC 07	ND	0.33	<0.005	0.04
BMSC 08	ND	0.42	<0.005	0.03
BMSC 09	ND	1.08	<0.005	0.04
BMSC 10	ND	0.82	<0.005	0.05
BMSC 11	ND	0.37	<0.005	ND
BMSC 12	ND	0.47	<0.005	ND
BMSC 13	ND	0.63	<0.022	ND
BMSC 14	ND	0.70	<0.005	ND

ND: not detected. Assessed in the cell supernatant during sample collection, BMSC isolation, and expansion (passages 0 and 2). Assessed in the supernatant from passage 2 BMSCs. Assessed in the supernatant from passage 2 BMSCs washed for 5 cycles with Ringer’s lactate solution containing 0.5% human albumin to reduce the residual level of bovine contaminants.