Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells

Figure 1

Effects of LY411575 on the osteoblast differentiation of hBMSCs. (a) Scatter plot depicting the initial ALP functional screen of 73 compounds for their effects on day 10 postosteoblastic differentiation of hBMSCs. -axis indicates the small molecule inhibitor, while each symbol represents replica. -axis indicates percent change in ALP activity compared to DMSO control-treated cells. (b) Representative alkaline phosphatase (ALP) staining of hBMSCs on day10 postosteoblastic differentiation, in the presence of LY411575 (3.0 μM) compared to DMSO-treated control cells. Images 10x magnification. Abbreviations: ALP: alkaline phosphatase; DMSO: dimethyl sulfoxide. (c) Quantification of ALP activity in hBMSCs following treatment with LY411575 (3.0 μM) versus DMSO vehicle-treated control cells on day 10 osteoblast differentiation. Data are presented as (). (d) Assay for cell number employing alamarBlue assay following treatment with LY411575 (3.0 μM) versus DMSO vehicle-treated control cells on day 10 postosteoblastic differentiation.

(a)

(b)

(c)

(d)