Research Article

Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells

Figure 2

LY411575 inhibits mineralized matrix formation of hBMSCs. (a) Cytochemical staining for mineralized matrix formation using Alizarin red stain performed on day 21 postosteoblastic differentiation in the absence (A) or presence (B) of LY411575 (3.0 μM). Magnification 10x. (b) Quantitative RT-PCR analysis for the gene expression of ALP, COL1A1, ON, and RUNX2 in hBMSCs on day 21 postosteoblast differentiation in the absence (blue) or presence (red) of LY411575 (3.0 μM). Gene expression was normalized to β-actin. Data are presented as (). . Abbreviations: ALP: alkaline phosphatase; COL1A1: collagen type I alpha 1; ON: osteonectin; RUNX2: runt-related transcription factor 2; DMSO: dimethyl sulfoxide.
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