Stem Cells International / 2019 / Article / Tab 1

Research Article

Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells

Table 1

List of antibodies and dilution for immunofluorescence staining.

Immunofluorescence antibodySpecificityDilutionCompanyCat. No.

CD31Mouse1 : 300R&DBBA7
NPHS1Rabbit1 : 200InvitrogenPA5-20330
NPHS2Rabbit1 : 400Proteintech20384-1-AP
PAX8Rabbit1 : 200Cell Signaling59019
PDGFR-βRabbit1 : 100Cell Signaling3169
SIX2Rabbit1 : 200Proteintech11562-1-AP
VimentinRabbit1 : 200Cell Signaling5741
Alexa 488Rabbit1 : 500InvitrogenA-11034
Alexa 555Rabbit1 : 500InvitrogenA-21428
Cy3Mouse1 : 500InvitrogenA10521
NANOGRabbit1 : 800Cell Signaling4903T
SSEA4Mouse1 : 1000Cell Signaling4755T
TRA-1-81Mouse1 : 1000Cell Signaling4745T

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