Research Article
Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells
Table 1
List of antibodies and dilution for immunofluorescence staining.
| | Immunofluorescence antibody | Specificity | Dilution | Company | Cat. No. |
| | CD31 | Mouse | 1 : 300 | R&D | BBA7 | | NPHS1 | Rabbit | 1 : 200 | Invitrogen | PA5-20330 | | NPHS2 | Rabbit | 1 : 400 | Proteintech | 20384-1-AP | | PAX8 | Rabbit | 1 : 200 | Cell Signaling | 59019 | | PDGFR-β | Rabbit | 1 : 100 | Cell Signaling | 3169 | | SIX2 | Rabbit | 1 : 200 | Proteintech | 11562-1-AP | | Vimentin | Rabbit | 1 : 200 | Cell Signaling | 5741 | | Alexa 488 | Rabbit | 1 : 500 | Invitrogen | A-11034 | | Alexa 555 | Rabbit | 1 : 500 | Invitrogen | A-21428 | | Cy3 | Mouse | 1 : 500 | Invitrogen | A10521 | | NANOG | Rabbit | 1 : 800 | Cell Signaling | 4903T | | SSEA4 | Mouse | 1 : 1000 | Cell Signaling | 4755T | | TRA-1-81 | Mouse | 1 : 1000 | Cell Signaling | 4745T |
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