Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker

<div>Multilineage differentiation assay of SHED-ori P4, SHED-ori P20, and SHED-Bmi1-EGFP P40. (a) Alizarin Red staining was performed to evaluate the osteogenic differentiation ability of the cells, and the semiquantitative results were calculated. (b) ALP staining and ALP activity assays were performed both in SHED-ori P4, SHED-ori P20, and SHED-Bmi1-EGFP P40. (c) Adipose droplets in cells were stained orange by Oil Red O after 21 days of adipogenic differentiation induction, and the semiquantitative results were calculated. (d) Osteogenic and adipogenic differentiation related genes were detected by qRT-PCR. Scale bar, 200 <i>μ</i>m. 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Stem Cells International

fig3

Figure 3

Figure 3: Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker 