Stem Cells International / 2019 / Article / Fig 3

Research Article

Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells

Figure 3

Epidermal stem cells developed into glandular cell clusters by coculture with EPP tissue. Epidermal stem cells were induced into glandular cell clusters by coculture with EPP tissue. Nonglandular or “sea snail” cell clusters were formed in the group with embryonic dorsal skin. (a) Morphological alterations of epidermal stem cells in different inducing development systems were observed by inverted microscope (all μm). (b) Histological features of multicellular clusters were evaluated by H&E staining in different inducing development systems (μm and 50 μm). (c) Specific biomarkers of sweat gland development were detected by immunofluorescent staining in different inducing development systems. All the nuclei were counterstained with DAPI (DAPI: blue; K5: green; and K14, K8, and K18: red) (μm). (K5: cytokeratin 5, K14: cytokeratin 14, K8: cytokeratin 8, and K18: cytokeratin 18).
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