Research Article

Human Embryonic Stem Cells Acquire Responsiveness to TRAIL upon Exposure to Cisplatin

Figure 3

A sublethal dose of cisplatin modulates the expression of death receptors and initiator caspases. Cells treated with 1 μM and 2 μM doses of cisplatin for 24 hours were assessed for the expression of key apoptotic signaling components and pluripotency-associated markers. (a) Western blot analysis of pro- (Bax) and antiapoptotic proteins (XIAP, Bcl-xL, and Mcl-1) involved in the regulation of the intrinsic apoptotic pathway. Staining with 0.1% amidoblack was used to evaluate the protein loading. Both CCTL12 and CCTL14 lines of hESC were used (). (b) Expression of cell membrane-linked death receptors with an affinity to TRAIL and (c) expression of pluripotency-associated membrane markers, as determined by flow cytometry. At least 10,000 cells were analyzed per sample. Red line—isotype control, green line—control cells without cisplatin, blue line—cells treated with 1 μM cisplatin, and brown line—cells treated with 2 μM cisplatin. Both CCTL12 and CCTL14 lines of hESC were used (); a representative picture is shown.
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