Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
Characteristics of rat bone marrow cells grown on AC-GlcNAc-coated and uncoated tissue-culture dishes. (a) Flow cytometric analysis of expression of cell-surface vimentin in rat bone marrow cells. (b) Phase-contrast images of colonies formed on tissue-culture dishes and AC-GlcNAc-coated dishes at 10 days. Lower images are enlargements of upper images. (c) Images of crystal violet-stained colonies and areas of colonies over 3 to 16 days of culture. ,.