Stem Cells International / 2019 / Article / Fig 3

Research Article

The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

Figure 3

The expression of TRAIL in AFMSCs was initiated by both IFN-β and IFN-γ, but only IFN-γ initiated the expression of IDO1 in AFMSCs. (a) AFMSCs were primed for 12 hours with IFN-β or IFN-γ (20 ng/ml each). TRAIL and IDO1 mRNA were measured by real-time PCR, and GAPDH was used as an internal control. The expression levels were normalized to the level of GAPDH mRNA (defined as an arbitrary unit). (b) AFMSCs were primed with IFN-β or IFN-γ at specified concentrations for 12 hours. The protein levels of TRAIL and IDO1 were determined by western blotting analysis. Results were representative of three independent experiments (, and , ).

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