Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications

Figure 1

Overview of experiments. (a1) Microwell plates were seeded to form aggregates of varying cells/microwell to control the aggregate size that best matched the aggregates formed in bioreactors from single cells. (a2) Single cells and preformed aggregates were cultured in suspension bioreactors and compared to statically cultured aggregates. (b) Microwell plates were seeded to form aggregates of varying cells/microwell to investigate the dependence of macromolecule deposition on initial aggregate cell number.

(a)

(b)