Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications

Figure 2

Analyses of microwell aggregates inoculated at 500, 1000, 1500, and 2000 cells/microwell after overnight formation. (a) Photomicrographs of aggregates in the microwells after overnight formation (). (b) Average diameter of the aggregates formed in the microwell plates. Significance is denoted by alphabetical lettering; groups with no significance are linked by the same letters, while groups with significance do not share the same letters (). (c) Average volume of the aggregates formed in the microwell plates. Linear regression given by and . (d) Distribution of aggregate diameter based on the number of cells/aggregate. At each cell density, triplicate wells were inoculated and 100 aggregates from each well were individually sized in two perpendicular directions ().

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