Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications

Figure 3

Cell proliferation in digested tissue-like aggregates as shown by (a) quantified DNA and (b) cell-fold expansion. DNA quantified using CyQUANT Cell Proliferation Assay Kit (Life Technologies). DNA in diploid primary cell assumed to be 6.6 pg/cell. Duplicate samples were harvested from the three bioreactors for the dynamic conditions and two wells from the static conditions, then subjected to quantification. Significance between culture time points (within conditions) is denoted by different alphabetical letters (). Significance between conditions is denoted by the presence of asterisks (, , , and ).

(a)

(b)