Kupffer Cells Promote the Differentiation of Adult Liver Hematopoietic Stem and Progenitor Cells into Lymphocytes via ICAM-1 and LFA-1 Interaction
The ability of adult liver LSK cells to differentiate into lymphocytes and myeloid cells in vivo. (a, c, e) liver or BM LSK cells obtained from CD45.2 mice were mixed with unfractionated CD45.1+ competitor bone marrow cells and intravenously injected into lethally irradiated CD45.1 recipient mice. Peripheral blood was collected weekly. The proportion of differentiated lymphoid (CD3+, CD19+, and NK1.1+) and myeloid (CD11b+) lineages of CD45.2+ cells in the peripheral blood of CD45.1+ recipient mice was detected by FACS at weeks 3, 6, and 9. The experiments were repeated three times. (b, d, f) Statistical chart of the proportion of CD3+ T, CD19+ B, NK1.1+ NK, and CD11b+ myeloid cells derived from CD45.2+ donor cells in the peripheral blood of CD45.1+ recipient mice at weeks 3, 6, and 9 (). Bars represent the of three independent experiments. . (g) Flow cytometric plots show the percentages of CD45.2+ cells in the liver of CD45.1+ recipient mice detected by FACS at the 9th week. The proportions of differentiated lymphoid (CD3+, CD19+, and NK1.1+) and myeloid (CD11b+) lineages of CD45.2+ cells in the liver of CD45.1+ recipient mice were detected by FACS.
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