Kupffer Cells Promote the Differentiation of Adult Liver Hematopoietic Stem and Progenitor Cells into Lymphocytes via ICAM-1 and LFA-1 Interaction

Figure 5

Kupffer cells can promote liver LSK cell proliferation. (a) A schematic map of the kupffer and LSK cell coculture. Freshly isolated kupffer cells were stimulated with 1 mg/mL LPS for 6 h, then cocultured with freshly sorted LSK cells for 24 h. (b) Statistical analysis of the percentage and absolute number of LSK cells among the mononuclear cells in the coculture system with or without LPS treatment. (c–f) An LPS-induced liver extramedullary hematopoiesis model was established with or without kupffer cell depletion. The proliferation of LSK cells in the liver and bone marrow was determined by Ki-67 labeling in the different treatment groups by flow cytometry. from three independent experiments are presented (). ; ; .

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