MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells

Figure 6

Formation of CFU-G, CFU-GM, and CFU-M colonies in myeloid CFU selective media. Wild-type (p38α) and mutant (p38α) 6-, 10-, and 14-day-old EBs were dissociated into single cells by trypsin and seeded for 14 days into a medium selective for myeloid cells. The frequencies of CFU-G (a), CFU-GM (b), and CFU-M (c) colonies derived from wild-type and mutant p38α EBs are shown. Data are presented as from a minimum of four independent experiments. Statistical significance was determined by ANOVA with post hoc Bonferroni’s multiple comparison test; asterisk “” indicates a statistical significance of .

(a)

(b)

(c)