Construction and Evaluation of a Subcutaneous Splenic Injection Port for Serial Intraportal Vein Cell Delivery in Murine Disease Models

Figure 2

Cell distribution following intrasplenic cell injection. (a) Illustration of mouse liver lobes and flow of the injected cells via the splenic vein to branches of the portal vein: left lateral lobe (LLL), middle left lobe (MLL), middle right lobe (MRL), right lateral lobe (RLL), and caudate lobe (CL). (b) Phase-contrast image shows the morphology of the immortalized human amniotic epithelial cells (hAECs). (c) The histogram of FACS prior to sorting. (d) Fluorescent image demonstrating the green fluorescent protein- (GFP-) labeled immortalized hAECs after sorting. (e) Cell distribution pie chart. The left chart demonstrates the ratio of human DNA to total DNA from each tissue. The right chart demonstrates the ratio of adjusted human DNA quantity in each liver lobe. (f) A dot plot demonstrating the correlation of the size of each lobe and adjusted quantity of human DNA in each lobe.

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