Construction and Evaluation of a Subcutaneous Splenic Injection Port for Serial Intraportal Vein Cell Delivery in Murine Disease Models

Figure 4

Multiple human amniotic epithelial cell injections. (a) The schematic shows an overview of the multiple cell injection study design. One week before the first cell transplantation, the subcutaneous cell injection port construction surgery was completed. A total of 1.5 million hAECs were transplanted every week for four times. The final cell injection was performed with GFP-labeled hAECs to confirm the function of the splenic injection port. (b) The low magnification fluorescent image (×10) of the recipient liver one hour after the final GFP-positive cell injection. The yellow arrows indicate the cells located in the area of small distal portal branches. (c) The cell distribution and migration are similar to those of the first cell transplantation after serial cell injections. DAPI (blue) and Alexa Fluor 594 Phalloidin (red) were used as counterstains to visualize nuclear and liver structures, respectively.

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