Research Article

Metformin Delays Satellite Cell Activation and Maintains Quiescence

Figure 10

In vitro metformin-treated SCs retain stem cell characteristics. (a) Dot plot of flow cytometric analysis of apoptotic cells after metformin treatment in vitro. Cell populations: alive cells (annexin V negative, PI negative), early apoptotic cells (annexin V positive, PI negative), late apoptotic cells (annexin V positive, PI positive), and necrotic cells (annexin V negative, PI positive). The position of the quadrant lines was stabilized based on the distinguishing cell populations. (b) Quantitation of the percentages of alive, early apoptotic, late apoptotic, and necrotic cells (n experimental ). (c) Representative histogram of the forward scattering (FSC) parameter by FACS analysis in control and metformin-treated SCs. (d) Representative histogram of pyronin Y FACS analysis in control and metformin-treated SCs.
(a)
(b)
(c)
(d)