Metformin Delays Satellite Cell Activation and Maintains Quiescence

Figure 8

Sirt1 levels in metformin-treated SCs. (a) Western blot analysis for the expression of Sirt1 and acH3 in control and metformin-treated SCs after 2, 4, 6, and 8 days of treatment (t2, t4, t6, and t8, respectively). Vinculin was used as a loading control. (b) Quantitation graph of Sirt1 protein levels monitored by western blot in four independent cell isolations and biological replicates (). Statistical significance was evaluated by the ANOVA test (). (c) Quantitation graph of acH3 protein levels monitored by western blot in four independent cell isolations and biological replicates (). Statistical significance was evaluated by the ANOVA test ().

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