Colony Formation, Migratory, and Differentiation Characteristics of Multipotential Stromal Cells (MSCs) from “Clinically Accessible” Human Periosteum Compared to Donor-Matched Bone Marrow MSCs

Figure 2

Quantification of MSC content, colony formation, and in vitro proliferation of periosteum and bone marrow-derived MSCs. (a) Colonies formed by MSCs during CFU-F assays. (b) Percentage MSCs in nucleated cell count. (c) Quantification of colony surface area () distribution (Gaussian distribution). (d) Mean colony size (). (e) In vitro proliferation of MSCs, calculated by cumulative population doublings, showing two distinct growth curves prior to passage (<P0) and post passage (>P0). (f) Days per population doubling compared from P0 to P4. (g) Flow cytometry histograms for positive and negative MSC markers. Statistical analysis was carried out, Wilcoxon signed-rank test (a), paired Student’s -test (d), Kruskal-Wallis test (comparison of P0 to P1-P4) , (f), and Wilcoxon signed-rank test (comparison of bone marrow to periosteum) (f). BM: bone marrow (black), P: periosteum (grey), NCC: nucleated cell, PD: population doubling.

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