Human Liver-Derived Stem Cells Improve Fibrosis and Inflammation Associated with Nonalcoholic Steatohepatitis

Figure 3

Gene expression profile of HLSCs. (a) Scatter plot showing the comparison of gene expression, screened by human MSC PCR array (PAHS-082Z) which includes genes that define the stemness, pluripotency, and self-renewal characteristics of MSCs and HLSCs at passage 3. The array does not include epithelial markers. Red dots represent genes more expressed by HLSCs, while blue dots represent genes more expressed by MSCs. Black dots are genes not differentially expressed. (b) Relative quantification (RQ) of genes more expressed by HLSCs with respect to MSCs. (c, d) Real-time PCR of hepatocyte growth factor (HGF) and cytokeratin-18 (CK18) expression in HLSCs compared with MSCs. Data are expressed as relative quantification using the ΔΔCt method. Normalization was made using TBP as a housekeeping gene. ANOVA with the Newman-Keuls multicomparison test was performed; HLSCs versus MSCs. (e, f) Scatter plots comparing the gene expression of HLSCs at passage 3 versus passage 6 (e) and passage 12 (f). Red dots represent genes more expressed by HLSCs at passage 6 or 12, while blue dots represent genes more expressed by HLSCs at passage 3. Black dots are genes not differentially expressed.

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