Pretreatment with AM1241 Enhances the Analgesic Effect of Intrathecally Administrated Mesenchymal Stem Cells
Expression of p-ERK following administration of exogenous TGF-β1. (a) The reversal of CCI-induced mechanical allodynia and (b) thermal hyperalgesia at 3 h after i.t. injection of exogenous TGF-β1. (c) Timeline shows the time of TGF-β1 treatment (CCI day 5) and tissue collection (3 h after injection). Arrows indicate the time of exogenous TGF-β1 injection. Inhibition of CCI-induced upregulation of p-ERK1/2 by i.t. injection of exogenous TGF-β1 (CCI day 5) via WB in the (d, e) DRGs and in the (f, g) spinal cord; mice/group. (h–k) Immunohistofluorescence analysis of p-ERK1/2 after i.t. injection of exogenous TGF-β1 (CCI day 5) in the (h, i) DRGs and in the (j, k) spinal dorsal horn; mice/group. Quantification results of p-ERK1/2 staining in the (i) DRGs and in the (k) spinal dorsal horn. Scale bar, 50 μm. , , and versus the sham group; , , and versus the CCI group.