MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing

Figure 2

Systemically infused MSCs skew macrophages to M2. (a) Dual-color immunofluorescence staining of CD68 (green) and RELM-α (red) at the wound site after systemic injection of BMMSCs and JMMSCs at days 3, 6, 9, and 12 (a1). Cell nuclei were counterstained with DAPI (blue). Comparison of the percentage of RELM-α and CD68 dual-positive macrophages (a2) (). (b) Western blot analysis of RELM-α expression in the wound samples and the surrounding normal skin samples after systemic injection of BMMSCs and JMMSCs. (c) Dual-color immunofluorescence staining of CD14 (green) and CD163 (red) in macrophages after being cocultured with BMMSCs or JBMMSCs. (d) The percentage of CD206-positive cells in macrophages after coculture with BMMSCs or JMMSCs by flow cytometry (). (e) qRT-PCR analysis of IL-10 and TNF-α in macrophages after being cocultured with BMMSCs or JBMMSCs (). Scale bars: 100 μm (a, c). , , and . Error bars are .

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