A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells

Figure 1

The phenotype and differentiation potential of mMSC-ATs in culture. The morphological appearance of mMSC-ATs (P0) ((a) left panel; scale bar = 800 μm). The results of flow cytometry of the cell surface markers of mMSC-ATs (P0) ((a) right panels). Representative images of adipocyte ((b) left panel; scale bar = 100 μm) and osteocyte differentiation ((b) right panel; scale bar = 200 μm) of mouse MSC-ATs (P0) cultured in differentiation medium. The morphological appearance of mMSC-ATs (P3) ((c) left panel; scale bar = 800 μm). The results of flow cytometry of the cell surface markers of mMSC-ATs (P3) ((c) right panels). Representative images of adipocyte ((d) left panel; scale bar = 100 μm) and osteocyte differentiation ((d) right panel; scale bar = 200 μm) of mouse MSC-ATs (P3) cultured in differentiation medium.

(a)

(b)

(c)

(d)