|
| Gene | Primer sequence (5-3) | Annealing temperature (°C) | Cycle | Product size (bp) |
|
| GAPDH | Forward: GTGGTCTCCTCTGACTTCAACA | 62 | 24 | 210 |
| Reverse: CTCTTCCTCTTGTGCTCTTGCT |
| AhR | Forward: AGTCTGTTATAACCCAGACCAG | 58 | 35 | 307 |
| Reverse: GCATCACAACCAATAGGTGTGA |
| Oct-4 | Forward: ACACTCGGACCACGTCTTTC | 54 | 32 | 300 |
| Reverse: CGTTCTCTTTGGAAAGGTGTTC |
| Nanog | Forward: ATAGCAATGGTGTGACGCAG | 62 | 32 | 219 |
| Reverse: GATTGTTCCAGGATTGGGTG |
| Sox-2 | Forward: AACCAAGACGCTCATGAAGAAG | 62 | 32 | 341 |
| Reverse: GCGAGTAGGACATGCTGTAGGT |
| SH-3(CD73) | Forward: TATTGCACTGGGACATTCGGGT | 62 | 35 | 443 |
| Reverse: GGTTGCCCATGTTGCATTCTCT |
| SH-2(CD105) | Forward: CATCCTTGAAGTCCATGTCCTCTT | 62 | 32 | 95 |
| Reverse: GCCAGGTGCCATTTTGCTT |
| HLA-G | Forward: GCGGCTACTACAACCAGAGC | 57 | 35 | 338 |
| Reverse: GCACATGGCACGTGTATCTC |
| TGF-β1 | Forward: GAGGTGACCTGGCCACCATT | 55 | 32 | 194 |
| Reverse: TCCGCAAGGACCTCGGCTGG |
| Dlx5 | Forward: ACCATCCGTCTCAGGAATCG | 60 | 32 | 384 |
| Reverse: ACCTTCTCTGTAATGCGGCC |
| Runx2 | Forward: TTGCAGCCATAAGAGGGTAG | 58 | 32 | 470 |
| Reverse: GTCACTTTCTTGGAGCAGGA |
| BMP7 | Forward: CCAACGTCATCCTGAAGAAATAC | 60 | 32 | 271 |
| Reverse: GCTTGTAGGATCTTGTTCATTGG |
| Sox9 | Forward: GGTTGTTGGAGCTTTCCTCA | 61 | 32 | 400 |
| Reverse: TAGCCTCCCTCACTCCAAGA |
| PPARG | Forward: TCTCTCCGTAATGGAAGACC | 55 | 32 | 474 |
| Reverse: GCATTATGAGACATCCCCAC |
| CYP1A1 | Forward: TCACAGACAGCCTGATTGAG | 58 | 35 | 434 |
| Reverse: GATGGGTTGACCCATAGCTT |
| C/EBPA | Forward: CCAAGAAGTCGGTGGACAAGAA | 62 | 32 | 145 |
| Reverse: TCATTGTCACTGGTCAGCTCCA |
|
