Research Article

Palmitic Acid Methyl Ester Induces G2/M Arrest in Human Bone Marrow-Derived Mesenchymal Stem Cells via the p53/p21 Pathway

Figure 1

Detection of FAMEs in rBM and effects of FAMEs on cell proliferation in rBM-MSCs and hBM-MSCs. Calibration curves were generated for quantification of PAME (a) and SAME (b) using methanol solutions containing PAME and SAME in five different concentrations (1 μM, 5 μM, 10 μM, 50 μM, and 100 μM). Both flushing PBS and flushing medium contain PAME (c) and SAME (d) from rBM (). PAME (10-100 μM), but not SAME, concentration-dependently inhibited proliferation of rBM-MSCs (-9) (e) and hBM-MSCs (-15) (f). All data represent . , versus the control group. Con: control; rBM: rat bone marrow; Veh: vehicle.
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