Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA

<div>Schematic representation of RNA constructs and quality control of DNA templates and synthesized RNA. The srRNA and synthetic modified mRNAs were synthesized using in vitro transcription (IVT). (a) Agarose gel electrophoresis showed the expected lengths for mRNAs encoding the reprogramming factors (Klf4: 1.6 kb; cMyc: 1.5 kb; Oct4: 1.3 kb; Sox2: 1.1 kb; and Lin28: 0.8 kb) and eGFP (0.9 kb). (b) The srRNA contains encoding sequences for the nonstructural proteins (nsP1-4); the reprogramming factors Oct4, Klf4, Sox2, and cMyc; and GFP. Agarose gel electrophoresis showed the expected length (17.7 kb) of linearized DNA and srRNA.</div>

Stem Cells International

fig1

Figure 1

Figure 1: Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA 