Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA

<div>RNA-based iPSC reprogramming of fibroblasts: srRNA vs. mRNA. (a) Emerging of iPSCs over time and detection of GFP-expressing cells during the reprogramming period. Phase contrast and fluorescence microscopic pictures are shown. (b) Live-antibody staining of obtained iPSC colonies. The iPSCs obtained after 20 days of reprogramming by mRNAs were positively stained with DL550 SSEA-4 and DL488 TRA-1-60 antibodies. iPSCs obtained after 12 days of reprogramming by srRNA were positive for SSEA-4 and showed only partial GFP expression within the iPSC colony. Scale bars represent 100 <i>μ</i>m.</div>

Stem Cells International

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Figure 3: Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA 