Research Article

Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA

Figure 4

Analysis of pluripotency markers in iPSCs generated by mRNA or srRNA delivery. (a) iPSCs stained with antibodies specific for pluripotent stem cell markers (Nanog, SSEA-4, TRA-1-60, Oct4, Sox2, and Lin28) showed a strong protein expression (). Scale bars represent 50 μm. (b) Analysis of Nanog and TRA-1-60 expression by flow cytometry. Data are shown as (). Statistical differences were determined using a paired -test (, ). (c) Analysis of Oct4, Sox2, Nanog, Lin28, and E-cadherin expression in iPSCs using qRT-PCR. mRNA levels were normalized to GAPDH mRNA levels, and the expression is presented relative to the expression levels in fibroblasts. Data are shown as (). Statistical differences were determined using a paired -test (, , and ).
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