Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists

Table 2

Effects of LPS and Poly(I:C) on the secretory capacity of BM-MSCs; a literature review summary.

Cell type (source)	Agonist (dose and exposure time)	Secreted proteins induced by LPS	Secreted proteins induced by Poly(I:C)	References
rBM-MSCs (Sprague Dawley rats) cultured on titanium surface	LPS (1 μg/mL for 6–72 h)	TNF-α, IL-1β	NA	[24]
rBM-MSCs (Sprague Dawley rats)	LPS (1 μg/mL for 6 h)	TNF-α, IL-1β	NA	[25]
mBM-MSCs (C57BL/6J mice)	LPS or Poly(I:C) (20 μg/mL for 24–72 h or 10 ng/mL–20 μg/mL for 7 days)	IL-6	IL-6	[15]
mBM-MSCs (C57BL/6J mice)	LPS (100 ng/mL for 24 h)	IL-6, VEGF-A	NA	[73, 74]
mBM-MSCs (C57BL/6 mice)	LPS (1 μg/mL for 48 h) or Poly(I:C) (20 μg/mL for 48 h)	IL-6	IL-6	[56]
mBM-MSCs (C57BL/10J mice)	LPS (0.01 to 10 μg/mL for 48 h)	VEGF	NA	[22]
mBM-MSCs (C57BL/6J mice)	LPS (1 μg/mL for 24 h) or Poly(I:C) (50 μg/mL for 24 h)	IL-6, IL-1β, IFN-γ	IL-6, TGF-β, IL-10, IL-1β, and IFN-β	[58]
mBM-MSCs (C57BL/6J mice)	LPS (0.01–10 mg/mL for 24 h) or Poly(I:C) (0.01–10 mg/mL for 24 h)	IL-6	IL-6	[94]
mBM-MSCs (C57BL/6J mice)	LPS (1000 ng/mL for 3 days)	IL-6, IL-1β	NA	[95]
hMSCs	LPS (10 ng/mL for 24 or 48 h) or Poly(I:C) (1 μg/mL for 24 or 48 h)	IL-6, IL-8, IP-10, TNF-α	IL-10, IL-12	[19]
hMSCs	LPS (10 ng/mL for 1 h) or Poly(I:C) (1 μg/mL for 1 h)+48 h without stimuli	IL-6, IL-8	IP-10, RANTES	[20]
hBM-MSCs	LPS (10 μg/mL for 18 h) or Poly(I:C) (30 μg/mL for 18 h)	IL-6, iL-8, RANTES	IL-6, IL-8, RANTES	[96]
hBM-MSCs	LPS (10 μg/mL for 48 h)	IL-6, MCP-1, IL-8, TNF-α, GM-CSF, IL-1β, G-CSF, MIP-1β, IFN-γ, IL-17, and IL-4	NA	[97]
hBM-MSCs	Poly(I:C) (1 μM for 4–24 h)	NA	IL-6, IL-8, IFN type I	[63]
hBM-MSCs	LPS (1–1000 ng/mL for 48 h) or Poly(I:C) (0.02–20 μg/mL for 48 h)	IL-6, IL-8, RANTES, IL-1β	IL-6, IL-8, RANTES, IL-1β	[56]
hBM-MSCs	LPS (5 μg/mL for 24 h) or Poly(I:C) (50 μg/mL for 24 h)	IL-6	IL-6	[57]
hBM-MSCs	LPS (10 μg/mL for 18 h) or Poly(I:C) (30 μg/mL for 18 h)	IL-6	IL-6, IL-23	[16]
hBM-MSCs	LPS (100 ng/mL for 24 h) or Poly(I:C) (100 μg/mL for 24 h)	IL-6, IL-8, GM-CSF	IL-6, IL-8, GM-CSF, and IFN-β	[98]
hBM-MSCs	LPS (10 ng/mL for 4 h) or Poly(I:C) (1–5 μM/mL for 4 h)+24 h without stimuli	IL-6, IL-8, IP-10, RANTES, and IFN-α	IL-6, IL-8, IP-10, RANTES, and IFN-α	[99]
hBM-MSCs	Poly(I:C) (5 μg/mL for 24 h)	NA	IL-6, IP-10	[100]
hBM-MSCs	LPS (10 μg/mL for 48 h)	IL-6, GM-CSF, G-CSF, M-CSF, and IL-11	NA	[101]
hBM-MSCs	LPS (10 ng/mL for 24 h)	IL-6, VEGF, MCP-1, IL-8, IP-10, RANTES, GM-CSF, MIP-1α, MIP-1β, SDF-1α, IL-1RA, GRO-α, IL-1α, IL-2, IL-31, and eotaxin	NA	[77]

G-CSF: granulocyte colony-stimulating factor; GM-CSF: granulocyte-macrophage colony-stimulating factor; GRO-α: growth-regulated oncogene-α; hBM-MSCs: human bone marrow-derived mesenchymal stromal cells; hMSCs: human mesenchymal stromal cells (unclear origin); IFN: interferon; IL: interleukin; IP-10: interferon gamma-induced protein 10; LPS: lipopolysaccharide; mBM-MSCs: mouse bone marrow-derived mesenchymal stromal cells; MCP-1: monocyte chemoattractant protein-1; M-CSF: macrophage colony-stimulating factor; MIP: macrophage inflammatory protein; NA: not available; Poly(I:C): polyinosinic-polycytidylic acid; RANTES: regulated on activation, normal T cell expressed and secreted; rBM-MSCs: rat bone marrow-derived mesenchymal stromal cells; VEGF: vascular endothelial growth factor.