Derivation and characterization of iPSCs from human MGCs. (a-b) Characterization of human MGCs by immunostaining with glial (GS and Vimentin) and microglial (CD18 and Iba1) markers. Nuclei were counterstained with DAPI (blue). Note the absence of staining with microglial markers as expected. (c) Positive alkaline phosphatase staining of human iPSC-5f derived from human MGCs at P10. (d-e) Immunofluorescence of pluripotency markers (SSEA4, OCT4, TRA1-81, and SOX2) for iPSC-5f at P15. (f) qRT-PCR analysis of pluripotency and self-renewal markers in human ESCs, iPSC-5f, and human MGCs. Data are normalized to human ESCs (scale bars: a, b, d, e, 60 μm; c, 200 μm).