Reprogramming of Adult Retinal Müller Glial Cells into Human-Induced Pluripotent Stem Cells as an Efficient Source of Retinal Cells

<div>Generation of pseudolaminated retinal organoids containing all retinal cell types from human MGC-derived iPSCs. (a-f) Immunofluorescence staining of cryosections from retinal organoids at D56 (a-c) and D100 (d-f) using markers for retinal ganglion cells (BRN3A, PAX6), horizontal cells (LHX1, PAX6), amacrine cells (AP2, PAX6), and photoreceptors (CRX, RCVRN). (g-i) Immunofluorescence staining of cryosections from retinal organoids at D150 (g) and D175 (h, i) using markers for photoreceptors (CRX), bipolar cells (VSX2 and PKC<i>α</i>), and MGCs (GS and SOX9). Nuclei were counterstained with DAPI (blue) (scale bars: a, 100 <i>μ</i>m; b-i, 50 <i>μ</i>m).</div>

Stem Cells International

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Figure 3

Figure 3: Reprogramming of Adult Retinal Müller Glial Cells into Human-Induced Pluripotent Stem Cells as an Efficient Source of Retinal Cells 