Stem Cells International / 2019 / Article / Fig 4

Research Article

Activated B Lymphocyte Inhibited the Osteoblastogenesis of Bone Mesenchymal Stem Cells by Notch Signaling

Figure 4

The osteoblastogenesis of BMSCs cocultured with normal B lymphocytes and LPS-pretreated B lymphocytes. (a) ALP and Alizarin red staining of BMSCs cocultured with normal B lymphocytes. (b) ALP and Alizarin red staining of BMSCs cocultured with LPS-pretreated B lymphocytes. (c) ALP and Alizarin red staining of BMSCs cocultured with LPS-pretreated B lymphocytes and dexamethasone. Alizarin red staining: calcified matrix in red and mineralization nodules in the dark. (d) mRNA expression of Hey1, Hey2, Notch2, Notch4, Hes1, and Runx2 in BMSCs. Data are presented as the ; , , and (/group).
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