Activated B Lymphocyte Inhibited the Osteoblastogenesis of Bone Mesenchymal Stem Cells by Notch Signaling

Figure 5

The osteoblastogenesis of BMSCs cocultured with LPS-pretreated B lymphocytes and DAPT. (a) ALP and Alizarin red staining of BMSCs cocultured with LPS-pretreated B lymphocytes and DAPT. Alizarin red staining: calcified matrix in red and mineralization nodules in the dark. (b) mRNA expression of Hey1, Hey2, Notch2, Notch4, Hes1, and Runx2 in BMSCs cocultured with LPS-pretreated B lymphocytes and DAPT. Data are presented as the ; , , and (/group).

(a)

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