Mesenchymal Stromal Cells Are More Effective Than Their Extracellular Vesicles at Reducing Lung Injury Regardless of Acute Respiratory Distress Syndrome Etiology

Figure 5

Protein levels of mediators in lung tissue. Protein levels of tumor necrosis factor- (TNF-) α, interleukin- (IL-) 6, IL-10, keratinocyte chemoattractant (KC) (a murine IL-8 homolog), vascular endothelial growth factor (VEGF), and transforming growth factor- (TGF-) β in lung tissue homogenate from (a) pulmonary ARDS (ARDSp) and (b) extrapulmonary ARDS (ARDSexp) animals. ARDS was induced by administration of Escherichia coli lipopolysaccharide intratracheally (ARDSp) or intraperitoneally (ARDSexp). Control mice (C) received saline solution intratracheally (Cp) or intraperitoneally (Cexp). After 24 h, ARDSp and ARDSexp animals were further randomized to receive saline (50 μL, SAL), bone marrow-derived MSCs (10⁵, 50 μL), or EVs (10⁵, 50 μL), stimulated (MSC serum, EV serum) or not with serum (MSCs, EVs) obtained from ARDSp or ARDSexp animals. Boxes show the interquartile (P25-P75) range, whiskers denote the range (minimum-maximum), and the horizontal line represents the median of animals per group. Significantly different from the corresponding C group (). Significantly different from the corresponding ARDS group (). ^#Significantly different from the corresponding MSC group ().

(a)

(b)