Stem Cells International

Research Article

Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats

Table 1

The primer sequence of qRT-PCR.


Gene		Primer sequences

Jagged1	Forward	5-CGCTGTATCTGTCCACCTGGCTAT-3
Jagged1	Reverse	5-TCACTGGCACGATTGTAGCATTGG-3
Notch1	Forward	5-TGGAGACAGGCAACAGTGAGGAA-3
Notch1	Reverse	5-CTTGGCAGCATCTGAACGAGAGTAT-3
Notch2	Forward	5-TACCACAACGGCACAGGCTACT-3
Notch2	Reverse	5-TTCTGACAGCGGTTCTTCTCACAAG-3
Notch3	Forward	5-CTGACTGTCTGTTCCTGTCCTCCA-3
Notch3	Reverse	5-CACCAGCACATTCATCCACATCCT-3
Notch4	Forward	5-CTGAAGCGGATGAATGTCGGAGTG-3
Notch4	Reverse	5-AGCAGGAACACAGCCAGGACTT-3
Hes1	Forward	5-CGGACAAACCAAAGACAGCCTCT-3
Hes1	Reverse	5-TGCCTTCTCCAGCTTGGAATGC-3
Hey1	Forward	5-CCGACGAGACCGAATCAATAACAGT-3
Hey1	Reverse	5-TTCAGCCAGGCATTCCCGAAAC-3
Collagen II	Forward	5-CGCTCAAGTCGCTGAACAACCA-3
Collagen II	Reverse	5-ACCAGTTCTTCCGAGGCACAGT-3
Aggrecan	Forward	5-TGGCCTGCCTGACTTTAGTG-3
Aggrecan	Reverse	5-CCTGAACCACTGACGCTGAT-3
Sox9	Forward	5-ACCATCACGCGCTCGCAGT-3
Sox9	Reverse	5-TGCGCTGGGTTCATGTAGGT-3
GAPDH	Forward	5-AGTGCCAGCCTCGTCTCATAGA-3
GAPDH	Reverse	5-GCCTTGACTGTGCCGTTGAACT-3

Sox9: SRY-related high-mobility group box gene 9; Hes1: hairy and enhancer of split-1; Hey1: Hes-related family BHLH transcription factor with YRPW motif 1.