Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices

Figure 3

Stem cell-derived neurons innervated the edge of CN slices within 14 days. Stem cell-derived neurons were cocultured with rat CN slices with the VIII nerve (ventral side) facing towards them (a). Within 14 days of culture, stem cell-derived neurons extensively innervated the edge of CN slices in cocultures (b, red square). These stem cell-derived neurites (positive for human NFM (red) and NFH (blue)) (c–f) innervated the edge of the CN slices and were immunopositive for synaptic markers synapsin I (c, d) and VGLUT1 (e, f). Scale bar in (f) = 20 μm, applies to all panels (c)–(f).