Research Article

Notch Inhibition Promotes Differentiation of Liver Progenitor Cells into Hepatocytes via sox9b Repression in Zebrafish

Figure 3

Sox9b suppresses LPC-to-hepatocyte differentiation. (a) Experimental scheme illustrating the period of MTZ treatment and analysis stage (arrow). (b) Single-optical section images showing Hnf4a and Tp1:H2B-mCherry expression in regenerating livers at A30h. Arrows point to Hnf4a+ BEC-derived cells; arrowheads point to Hnf4a- BEC-derived cells. (c) Quantification of the percentage of Hnf4a+ cells among BEC-derived cells, as shown in (b). (d) Experimental scheme illustrating the periods of MTZ and 4-OHT treatment and analysis stage (arrow). (e) Single-optical section images showing fabp10a:CFP-NTR, Bhmt, and mCherry expression in regenerating livers at A24h. mCherry expression was revealed by anti-mCherry immunostaining. Arrows point to CFP+/Bhmt-/mCherry+ BEC-derived cells; arrowheads point to CFP+/mCherry-/Bhmt+ hepatocytes. (f) Quantification of the percentage of Bhmt+ hepatocytes between CFP+/mCherry- and CFP+/mCherry+ populations, as shown in (e). Scale bars: 100 μm; error bars: ±SEM. ; .
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