Figure 3: The expression of stem cell markers and HIF in spheroid-forming cells and monolayer-cultured cells. The relative expression of FUT4 (SSEA1) (a), Sox2 (b), Nanog (d), and HIF-2α (e) was significantly higher in spheroid-forming cells at any time point examined. The expression of Oct4 (c) in spheroids was significantly higher than that of monolayer-cultured cells at 72 hours. Data are represented as the . (a and b) . (c) . (d) . (e) . , , and .